![]() ![]() Further, the conjugates’ most frequently used detection techniques include Alkaline Phosphatase (AP) and Horseradish Peroxidase (HRP). Today, many detection methods of proteins are available, such as chemiluminescence, fluorescence, and calorimetric detection techniques. The target protein bands are then incubated with the primary antibody having epitope for the protein of interest followed by a secondary antibody conjugated with an enzyme or fluorescent molecules. The workflow involves first separating the proteins based on their molecular weight (in kDa) in the form of bands through sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) followed by transferring the bands on the nitrocellulose membrane or PVDF membrane using electric current (the process known as immunoblotting). It’s based on the interaction between target antigens of a protein sample with specific antibodies. The technique was first introduced by Towbin et al. Further, it’s also a crucial tool to study protein-protein interactions. It’s a powerful procedure to analyze the relative mass, presence, and relative abundance of post-translational modifications in proteins. If you think you may have a medical emergency, call your physician or 911 immediately.Western blotting is a powerful technique used in molecular biology and proteomics labs to study proteins obtained from a cell lysate or other protein samples. By using this Site you agree to the following Terms and Conditions. We offer this Site AS IS and without any warranties. Never disregard the medical advice of your physician or health professional, or delay in seeking such advice, because of something you read on this Site. We disclaim all responsibility for the professional qualifications and licensing of, and services provided by, any physician or other health providers posting on or otherwise referred to on this Site and/or any Third Party Site. ![]() MedHelp is not a medical or healthcare provider and your use of this Site does not create a doctor / patient relationship. It is not intended to be and should not be interpreted as medical advice or a diagnosis of any health or fitness problem, condition or disease or a recommendation for a specific test, doctor, care provider, procedure, treatment plan, product, or course of action. The Content on this Site is presented in a summary fashion, and is intended to be used for educational and entertainment purposes only. Do you have any other advice for me or should I just assume I'm negative and that all the bumps and tingles are related to something else? This is the link to my original thread: This discussion is related to Can I finally trust these test results?. I guess the "high negative" antibody levels on the IGg tests I had don't mean anything after all? I still have the weird bumps on my scalp and the intermittent scrotal tingling, but all the tests say it's not related to herpes. So, that's 5 negative IGg tests, 1 equivocal IGg, 1 positive IGm, and 1 negative Western Blot (after almost exactly a year since I've had sex and without antivirals for almost 7 months). He was a little surprised/stumped and thought it would definitely be positive after the equivocal IGg and the positive (1.90) IGm. My doctor called this morning and told me that the Western Blot from the University of Washington was negative for both types. Hi Terri- I started a discussion with you that is now closed, but I just wanted to quickly update the status of my situation. ![]()
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